University of Minnesota
https://twin-cities.umn.edu/
612-625-5000
Milestone
3.1.a

Potency assays

Completed

Streamline the production of new potency assays to ensure timely release of annual vaccine preparations.

Progress Highlights

Bodle 2023 demonstrated the applicability of the homologous mAb ELISA as a replacement for the SRID assay for HA antigen quantitation and stability assessment.

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Narayan 2023 developed a Surface Plasmon Resonance-based method using influenza subtype or lineage HA-specific mAbs to measure the HA concentration in multivalent influenza vaccines, which eliminates the need for annually produced antisera reagents. 

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Qian 2023 developed a streamlined subtype-specific, reference antigen and antisera-free method (CombE-IDMS) for rapidly assaying the potency of influenza vaccines as an alternate to the SRID assay.

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Cheung 2022 developed two ELISA-based potency assays for group 1 influenza A viruses using cross-reactive nanobodies. 

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Odon 2024 used a mammalian cell transfection method to produce recombinant HAs as immunogens for the SRID assay, which measures the potency of inactivated influenza vaccines. Results suggest that this method can be used to prepare a library of reagents for influenza vaccine potency assays. 

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